In this collaborative biothreat assessment exercise, students engage in a structured evaluation of public health threats using the European Centre for Disease Prevention and Control (ECDC) framework, employing a Multi-criteria Decision Analysis (MCDA) approach. Through this problem-based learning activity, students deepen their understanding of epidemiological principles by synthesizing theoretical knowledge with practical application. Utilizing the ECDC framework, students work in small groups to systematically assess factors such as pathogen characteristics, transmission dynamics, and potential impact on public health. The MCDA approach allows them to prioritize and weigh these criteria based on their significance and uncertainty. Through active collaboration, students not only develop critical thinking and analytical skills but also enhance their ability to work effectively in teams. By simulating real-world scenarios, this exercise prepares students to make informed decisions in public health emergencies, reinforcing the importance of rigorous assessment and evidence-based reasoning in epidemiology and biothreat management.