This session presents a transformative approach to education implemented in a large microbiology/STEM course (120+ students). By eliminating quizzes and exams as assessments, we shifted to utilizing e-portfolios to assess student learning. E-portfolios allow students to draw connections between the course material in creative ways. and enhances their critical thinking and application skills. Attendees will discuss various assessment strategies and learn how to design assignments that can be included in a portfolio. We will also share insights from our course transformation and highlight the impact on student learning and their overall engagement in the class.